CHEK2 Protein (GST tag)

Catalog No. ABIN7317121

Product Details

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This CHEK2 protein is labelled with GST tag.

Purpose: Recombinant Human CHK2/CHEK2 Protein (GST Tag)

Sequence: Met 1-Leu543

Characteristics: A DNA sequence encoding the human CHEK2 (NP_009125.1) (Met1-Leu543) was expressed with a GST tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 150 mM NaCl, 25 %glycerol, pH 7.5, 0.1 mM EDTA, 0.5 mM TCEP

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: CHK2/CHEK2 (CHEK2 Products)

Background:

Background: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by CHEK2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded CHEK2 protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in CHEK2s gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: CDS1;CHK2;hCds1;HuCds1;LFS2;PP1425;RAD53

Molecular Weight: 88.1 kDa

NCBI Accession: NP_009125

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle