CHEK2 Protein (AA 1-543) (GST tag)
(2 references)-
- Target See all CHEK2 Proteins
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-543
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This CHEK2 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- CHEK2 (Human) Recombinant Protein (P01)
- Sequence
- MSRESDVEAQ QSHGSSACSQ PHGSVTQSQG SSSQSQGISS SSTSTMPNSS QSSHSSSGTL SSLETVSTQE LYSIPEDQEP EDQEPEEPTP APWARLWALQ DGFANLECVN DNYWFGRDKS CEYCFDEPLL KRTDKYRTYS KKHFRIFREV GPKNSYIAYI EDHSGNGTFV NTELVGKGKR RPLNNNSEIA LSLSRNKVFV FFDLTVDDQS VYPKALRDEY IMSKTLGSGA CGEVKLAFER KTCKKVAIKI ISKRKFAIGS AREADPALNV ETEIEILKKL NHPCIIKIKN FFDAEDYYIV LELMEGGELF DKVVGNKRLK EATCKLYFYQ MLLAVQYLHE NGIIHRDLKP ENVLLSSQEE DCLIKITDFG HSKILGETSL MRTLCGTPTY LAPEVLVSVG TAGYNRAVDC WSLGVILFIC LSGYPPFSEH RTQVSLKDQI TSGKYNFIPE VWAEVSEKAL DLVKKLLVVD PKARFTTEEA LRHPWLQDED MKRKFQDLLS EENESTALPQ VLAQPSTSRK RPREGEAEGA ETTKRPAVCA AVL
- Characteristics
- Human CHEK2 full-length ORF ( AAH04207.1, 1 a.a. - 543 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Checkpoint Kinase 2 (CHEK2) (AA 1-100) protein (GST tag)
CHEK2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Checkpoint Kinase 2 (CHEK2) protein (GST tag)CHEK2 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Checkpoint Kinase 2 (CHEK2) protein (GST tag,His tag)CHEK2 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE
Checkpoint Kinase 2 (CHEK2) (AA 1-543) protein (Strep Tag)Crystallography grade CHEK2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Checkpoint Kinase 2 (CHEK2) (AA 1-546) protein (Strep Tag)Crystallography grade CHEK2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Checkpoint Kinase 2 (CHEK2) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)CHEK2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, PI, STD Active
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "NormaCurve: a SuperCurve-based method that simultaneously quantifies and normalizes reverse phase protein array data." in: PLoS ONE, Vol. 7, Issue 6, pp. e38686, (2012) (PubMed).
: "Improvement of protein immobilization for the elaboration of tumor-associated antigen microarrays: application to the sensitive and specific detection of tumor markers from breast cancer sera." in: Biosensors & bioelectronics, Vol. 40, Issue 1, pp. 385-92, (2012) (PubMed).
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: "NormaCurve: a SuperCurve-based method that simultaneously quantifies and normalizes reverse phase protein array data." in: PLoS ONE, Vol. 7, Issue 6, pp. e38686, (2012) (PubMed).
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- Target
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
- Alternative Name
- CHEK2 (CHEK2 Products)
- Synonyms
- CDS1 Protein, CHK2 Protein, HuCds1 Protein, LFS2 Protein, PP1425 Protein, RAD53 Protein, hCds1 Protein, fa66f08 Protein, wu:fa66f08 Protein, zgc:55865 Protein, Cds1 Protein, HUCDS1 Protein, Rad53 Protein, Chk2 Protein, cds1 Protein, chek2 Protein, chk2 Protein, hucds1 Protein, lfs2 Protein, pp1425 Protein, rad53 Protein, checkpoint kinase 2 Protein, serine/threonine-protein kinase chk2 Protein, checkpoint kinase 2 L homeolog Protein, CHEK2 Protein, chek2 Protein, Chek2 Protein, MCYG_07308 Protein, chek2.L Protein
- Background
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Full Gene Name: CHK2 checkpoint homolog (S. pombe)
Synonyms: CDS1,CHK2,HuCds1,LFS2,PP1425,RAD53 - Gene ID
- 11200
- Pathways
- p53 Signaling, Apoptosis, Cell Division Cycle
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