CHEK2 Protein (GST tag,His tag)
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- Target See all CHEK2 Proteins
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
- Protein Type
- Recombinant
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Origin
- Mouse
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This CHEK2 protein is labelled with GST tag,His tag.
- Purpose
- Recombinant Mouse CHK2/CHEK2 Protein (His & GST Tag)(Active)
- Sequence
- Mey 1-Leu 546
- Characteristics
- A DNA sequence encoding the mouse CHEK2 (Q9Z265) (Mey 1-Leu 546) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- Purity
- > 90 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Kinase activity untested
- Top Product
- Discover our top product CHEK2 Protein
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Checkpoint Kinase 2 (CHEK2) (AA 1-546) protein (His tag)
custom-made CHEK2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Checkpoint Kinase 2 (CHEK2) (AA 1-543) protein (His tag)custom-made CHEK2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Checkpoint Kinase 2 (CHEK2) (AA 1-543) protein (GST tag)CHEK2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Checkpoint Kinase 2 (CHEK2) protein (GST tag)CHEK2 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Checkpoint Kinase 2 (CHEK2) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)CHEK2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, PI, STD Active
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 8.0
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
- Alternative Name
- CHK2/CHEK2 (CHEK2 Products)
- Background
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Background: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by CHEK2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded CHEK2 protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in CHEK2s gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Synonym: Cds1;CHK2;HUCDS1;Rad53
- Molecular Weight
- 89 kDa
- UniProt
- Q9Z265
- Pathways
- p53 Signaling, Apoptosis, Cell Division Cycle
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