CNTF Protein (AA 2-200)

Catalog No. ABIN7319650

This Recombinant CNTF protein is expressed in Escherichia coli (E. coli).

Quick Overview for CNTF Protein (AA 2-200) (ABIN7319650)

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 2-200

Purpose: Recombinant Human CNTF Protein

Sequence: Ala2-Met200

Characteristics: Recombinant Human Ciliary Neurotrophic Factor is produced by our E.coli expression system and the target gene encoding Ala2-Met200 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 6 % Sucrose, 4 % Mannitol, 0.05 % Tween 80, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Alternative Name: CNTF

Background: CNTF,Ciliary Neurotrophic Factor,Ciliary Neurotrophic Factor (CNTF) is a potent survival factor for neurons and oligodendrocytes. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83 % homology in their protein sequence. CNTF is structurally related to IL6, IL11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal transducing subunit in their receptor complexes. CNTF, like FGF acidic, FGF basic, and PD-ECGF (platelet-derived endothelial cell growth factor), does not possess a signal sequence that would allow secretion of the factor by classical secretion pathways. The mechanism underlying the release of CNTF is unknown.

Molecular Weight:

Calculated MW: 22.9 kDa

Observed MW: 25 kDa

Gene ID: 1270

UniProt: P26441

Pathways: JAK-STAT Signaling