Tissue factor Protein (His tag)

Catalog No. ABIN7195150

This Recombinant Tissue factor protein is expressed in HEK-293 Cells.

Quick Overview for Tissue factor Protein (His tag) (ABIN7195150)

Target: Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 98 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This Tissue factor protein is labelled with His tag.

Purpose: Recombinant Mouse Tissue Factor/CD142 Protein (His Tag)(Active)

Sequence: Met 1-Glu 251

Characteristics: A DNA sequence encoding the extracellular domain of mouse F3 (NP_034301.3) (Met 1-Glu 251) was expressed, with a C-terminal polyhistidine tag.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC (R&D Systems, Catalog# ES011). The AC50 is < 5 μg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))

Alternative Name: Tissue Factor/CD142

Background:

Background: Tissue factor (TF), also known as coagulation factor III, F3, and CD142, is a single-pass type I membrane protein which belongs to the tissue factor family. Tissue factor is one of the proteins that participate in hemostatic and inflammatory processes. Activated monocytes present in the liver increase expression of tissue factor, and while accumulating in the organ they can intensify inflammation. Tissue factor is the protein that activates the blood clotting system by binding to, and activating, the plasma serine protease, factor VIIa, following vascular injury. Tissue factor is not only the main physiological initiator of normal blood coagulation, but is also important in the natural history of solid malignancies in that it potentiates metastasis and angiogenesis and mediates outside-in signalling. Tissue factor is expressed constitutively by many tissues which are not in contact with blood and by other cells upon injury or activation, the latter include endothelial cells, tissue macrophages, and peripheral blood monocytes. Coagulation Factor III is a transmembrane glycoprotein that localizes the coagulation serine protease factor VII/VIIa (FVII/VIIa) to the cell surface. The primary function of TF is to activate the clotting cascade. The TF:FVIIa complex also activates cells by cleavage of a G-protein coupled receptor called protease-activated receptor 2 (PAR2). TF is expressed by tumor cells and contributes to a variety of pathologic processes, such as thrombosis, metastasis, tumor growth, and tumor angiogenesis. As a key regulator of haemostasis and angiogenesis, it is also involved in the pathology of several diseases, including cardiovascular, inflammatory and neoplastic conditions.

Synonym: AA409063,CD142,Cf-3,Cf3,TF

Molecular Weight: 26.8 kDa

NCBI Accession: NP_034301

Pathways: Positive Regulation of Endopeptidase Activity, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling