CSF1R Protein (His-GST)

Catalog No. ABIN7317115

Recombinant CSF1R protein expressed in Baculovirus infected Insect Cells.

Quick Overview for CSF1R Protein (His-GST) (ABIN7317115)

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 82 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CSF1R protein is labelled with His-GST.

Purpose: Recombinant Human CSF1R/CD115 Protein (His & GST Tag)

Sequence: Lys 543-Cys 972

Characteristics: A DNA sequence encoding the human CSF1R (NP_005202.2) cytoplasmic domain (Lys 543-Cys 972) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 50 mM Tris, 150 mM NaCl, pH 7.4, 20 % glycerol, 0.3 mM DTT

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Alternative Name: CSF1R/CD115

Background: C-FMS;CD115;CSF-1 receptor;CSF-1-R;CSF-1R;CSF1R;FIM2;FMS;HDLS;M-CSF-R;MCSF Receptor ;Macrophage colony-stimulating factor 1 receptor;Proto-oncogene c-Fms,M-CSFR encoded by the proto-oncogene c-fms is the receptor for colony stimulating factor 1 (CSF1R), a cytokine involved in the proliferation, differentiation, and activation of macrophages. This cell surface glycoprotein is consisted by an extracellular ligand-binding domain, a single membrane-spanning segment, and an intracellular tyrosine kinase domain. Binding of CSF1 activates the receptor kinase, leading to "autophosphorylation" of receptor subunits and the concomitant phosphorylation of a series of cellular proteins on tyrosine residues. CSF1R is a tyrosine kinase receptor that is absolutely required for macrophage differentiation and thus occupies a central role in hematopoiesis. CSF1 and its receptor (CSF1R, product of c-fms proto-oncogene) were initially implicated as essential for normal monocyte development as well as for trophoblastic implantation. This apparent role for CSF1/CSF1R in normal mammary gland development is very intriguing because this receptor/ligand pair has also been found to be important in the biology of breast cancer in which abnormal expression of CSF1 and its receptor correlates with tumor cell invasiveness and adverse clinical prognosis. Tumor cell expression of CSF1R is under the control of several steroid hormones (glucocorticoids and progestins) and the binding of several bHLH transcription factors, while tumor cell expression of CSF-1 appears to be regulated by other hormones, some of which are involved in normal lactogenic differentiation.

Molecular Weight:

Calculated MW: 76 kDa

Observed MW: 75 kDa

Gene ID: 1436

NCBI Accession: NP_005202

UniProt: P07333

Pathways: RTK Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization