C5A Protein (AA 678-751)

Catalog No. ABIN7319677

This Recombinant C5A protein is expressed in Escherichia coli (E. coli).

Quick Overview for C5A Protein (AA 678-751) (ABIN7319677)

Target: C5A (C5a) (Complement Component 5a (C5a))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 678-751

Purpose: Recombinant Human Complement C5a Protein

Sequence: Thr678-Arg751

Characteristics: Recombinant Human C5a is produced by our E.coli expression system and the target gene encoding Thr678-Arg751 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: C5A (C5a) (Complement Component 5a (C5a))

Alternative Name: Complement C5a

Background: C5Da,C5a,C5a anaphylatoxin,CPAMD4,Complement C5,ECLZB,Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 α-chain. Human C5a has four α-helices plus three intrachain disulfide bonds that create a triple loop structure. Human C5a is 60 % and 54 % aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2. Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.

Molecular Weight:

Calculated MW: 8.4 kDa

Observed MW: 12 kDa

Gene ID: 727

UniProt: P01031

Pathways: Complement System, Carbohydrate Homeostasis