Cyclin E1 Protein (CCNE1) (His-GST)

Catalog No. ABIN7317726

Recombinant Cyclin E1 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for Cyclin E1 Protein (CCNE1) (His-GST) (ABIN7317726)

Target: Cyclin E1 (CCNE1)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Application: ELISA

Purity: > 82 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Cyclin E1 protein is labelled with His-GST.

Purpose: Recombinant Human CCNE1/Cyclin-E1 Protein (His & GST Tag)

Sequence: Met 1-Ala 410

Characteristics: A DNA sequence encoding the full length of human CCNE1 isoform 1 (NP_001229.1) (Met 1-Ala 410) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized human HG3C-CCNE1 at 10 μg/ml (100 μl/well) can bind biotinylated human GST-CDK4, The EC₅₀ of biotinylated human GST-CDK4 is 45.0-106.0 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, 1 mM GSH, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Cyclin E1 (CCNE1)

Alternative Name: CCNE1/Cyclin-E1

Background: CCNE,Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. Cyclin E1 serves as regulatory subunits that bind, activate, and provide substrate for its associated cyclin-dependent kinase2 (CDK2), whose activity is essential for cell cycle G1 / S transition. Over expression of this encoding gene has been found in many tumors, which results in chromosome instability and by extension, induce tumorigenesis. This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In general, cyclin E1, as an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression and is frequently overexpressed in cancer cells.

Molecular Weight:

Calculated MW: 75.0 kDa

Observed MW: 70 kDa

Gene ID: 898

NCBI Accession: NP_001229

UniProt: P24864

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases