LAP3 Protein (His tag)
Quick Overview for LAP3 Protein (His tag) (ABIN7317187)
Target
See all LAP3 ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This LAP3 protein is labelled with His tag.
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Purpose
- Recombinant Human LAP3/Cytosol Aminopeptidase Protein (His Tag)
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Sequence
- Met 1-Ala519
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Characteristics
- A DNA sequence encoding the human LAP3 (NP_056991.2) (Met1-Ala519) was expressed with a polyhistidine tag at the C-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Cytosol Aminopeptidase (LAP3) (AA 1-519) protein (His tag)
custom-made LAP3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cytosol Aminopeptidase (LAP3) (AA 1-519) protein (His tag)custom-made LAP3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cytosol Aminopeptidase (LAP3) (AA 1-519) protein (GST tag)LAP3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Cytosol Aminopeptidase (LAP3) (AA 252-368) protein (His-IF2DI Tag)LAP3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Cytosol Aminopeptidase (LAP3) (AA 1-519) protein (His-IF2DI Tag)LAP3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Cytosol Aminopeptidase (LAP3) (Active) protein (Myc-DYKDDDDK Tag)LAP3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
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Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 20 % glycerol, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- LAP3 (Cytosol Aminopeptidase (LAP3))
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Alternative Name
- LAP3/Cytosol Aminopeptidase
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Background
- HEL-S-106,LAP,LAPEP,PEPS,LAP3 (Leucine Aminopeptidase 3) is a Protein Coding gene. 2 alternative initiation human isoforms have been reported. LAP3, belonging to the peptidase M17 family, has been proved to catalyze the hydrolysis of leucine residues. It catalyzes the removal of unsubstituted N-terminal amino acids from various peptides and is presumably involved in the processing and regular turnover of intracellular proteins. Leucine aminopeptidases are involved in many pathological disorders and regulate cell proliferation, invasion, and/or angiogenesis of tumors. A recent study showed that LAP3 is highly expressed in several malignant and affects tumor angiogenesis. LAP3 is widely expressed in the appendix, lymph node, and other tissues. Diseases associated with LAP3 include Bacterial Vaginosis and Trichomoniasis.
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Molecular Weight
- 57.6 kDa
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Gene ID
- 51056
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NCBI Accession
- NP_056991
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UniProt
- P28838
Target
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