DAP Kinase 1 Protein (AA 1-363) (His-GST)
Quick Overview for DAP Kinase 1 Protein (AA 1-363) (His-GST) (ABIN7317057)
Target
See all DAP Kinase 1 (DAPK1) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 1-363
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Purification tag / Conjugate
- This DAP Kinase 1 protein is labelled with His-GST.
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Purpose
- Recombinant Human DAPK1/DAP Kinase 1 Protein (aa 1-363, His & GST Tag)
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Sequence
- Met 1-Leu 363
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Characteristics
- A DNA sequence encoding the N-terminal segment of human DAPK1 (P53355-1) (Met 1-Leu 363) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- The specific activity was determined to be 20 nmol/min/mg using synthetic R11-S6-Peptide (R11-IAKRRRLSSLRASTSKSESSQK) as substrate.
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Death-Associated Protein Kinase 1 (DAPK1) (AA 1-1442) protein (His tag)
custom-made DAPK1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Death-Associated Protein Kinase 1 (DAPK1) (AA 1-1430) protein (His tag)custom-made DAPK1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Death-Associated Protein Kinase 1 (DAPK1) (AA 1211-1310) protein (GST tag)DAPK1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Death-Associated Protein Kinase 1 (DAPK1) (AA 1-368) protein (GST tag)DAPK1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Death-Associated Protein Kinase 1 (DAPK1) protein (Myc-DYKDDDDK Tag)DAPK1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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Expiry Date
- 6 months
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- DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))
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Alternative Name
- DAPK1/DAP Kinase 1
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Background
- DAPK,Death-associated protein kinase 1, also known as DAP kinase 1, DAPK1 and DAPK, is a cytoplasm protein which belongs to theprotein kinase superfamily, CAMK Ser / Thr protein kinase family and DAP kinase subfamily. DAPK1 contains tenANK repeats, onedeath domain and oneprotein kinase domain. DAPK1 is a calcium / calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK1 gene is a candidate tumor suppressor (TSG) and the abnormal methylation of DAPK1 gene has been found in many carcinomas. DAPK1 over-expression can induce cell apoptosis and inhibit tumor cell metastasis. DAPK1 gene over-expression could suppress PGCl3 cells malignant phenotype, inhibit PGCl3 cells growth, invasive, migration and adhesion ability, upregulate p53 gene and downregulate bcl-2 gene. Loss of activity of death-associated protein kinase 1 ( DAPK1 ) may be an independent factor affecting survival of non-small cell lung cancer patients. DAPK1 promoter methylation might play a significant role in the progression of chronic myeloid leukemia ( CML ).
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Molecular Weight
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Calculated MW: 69.4 kDa
Observed MW: 64 kDa
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Gene ID
- 1612
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UniProt
- P53355
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Pathways
- MAPK Signaling, Interferon-gamma Pathway
Target
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