DCK Protein (His-T7)
Quick Overview for DCK Protein (His-T7) (ABIN7318399)
Target
See all DCK ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This DCK protein is labelled with His-T7.
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Purpose
- Recombinant Human Deoxycytidine Kinase/DCK Protein (His &T7 Tag)
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Sequence
- Met 1-Leu260
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Characteristics
- Recombinant Human Deoxycytidine Kinase is produced by our E.coli expression system and the target gene encoding Met1-Leu260 is expressed with a 6His, T7 tag at the N-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Deoxycytidine Kinase (DCK) (AA 1-260) protein (His tag)
custom-made DCK Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Deoxycytidine Kinase (DCK) (AA 1-260) protein (His tag)custom-made DCK Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Deoxycytidine Kinase (DCK) (AA 1-260) protein (GST tag)DCK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Deoxycytidine Kinase (DCK) (AA 1-260) protein (His tag)DCK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as a 0.2 μm filtered solution of PBS, 50 % Glycerol, 1 mM TCEP, pH 7.4.
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Preservative
- Without preservative
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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Expiry Date
- 6 months
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- DCK (Deoxycytidine Kinase (DCK))
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Alternative Name
- Deoxycytidine Kinase/DCK
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Background
- DCK,Deoxycytidine Kinase,dCK,Deoxycytidine Kinase (DCK) is a member of the DCK/DGK family. DCK exists as a homodimer and is localized to the nucleus. DCK is required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG), and deoxyadenosine (dA). DCK has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. In addition, DCK is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. DCK is clinically important because of its relationship to drug resistance and sensitivity.
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Molecular Weight
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Calculated MW: 34.0 kDa
Observed MW: 30 kDa
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Gene ID
- 1633
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UniProt
- P27707
Target
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