ENTPD5 Protein (His tag)

Catalog No. ABIN7195472

Recombinant ENTPD5 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for ENTPD5 Protein (His tag) (ABIN7195472)

Target: ENTPD5 (Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Baculovirus infected Insect Cells

Purity: > 90 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This ENTPD5 protein is labelled with His tag.

Purpose: Recombinant Mouse ENTPD5 Protein (His Tag)(Active)

Sequence: Thr19-Ser427

Characteristics: A DNA sequence encoding the mouse ENTPD5 (Q9WUZ9) (Thr19-Ser427) was fused with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to hydrolyze the 5'phosphate groups from the substrate guanosine 5'diphosphate (GDP).The specific activity is > 15000 pmols/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 3 mM DTT, 10 % glycerol, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ENTPD5 (Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5))

Alternative Name: ENTPD5

Background:

Background: Ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5), also known as CD39 antigen-like 4, ER-UDPase, Guanosine-diphosphatase ENTPD5, Nucleoside diphosphatase Uridine-diphosphatase ENTPD5. This hydrolase is expressed in response to phosphoinositide 3-kinase (PI3K) signaling. Activation of PI3K results in FOXO phosphorylation by AKT1 and loss of ENTPD5 transcriptional repression. It is Up-regulated in PTEN-deficient cells. Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation.ENTPD5 promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, ENTPD5 constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. ENTPD5 also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. This enzyme Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.

Synonym: AI196558,AI987697,Cd39l4,ER-UDPase,mNTPase,NTPDase-5,NTPDase5,Pcph

Molecular Weight: 46.6 kDa

UniProt: Q9WUZ9