ENTPD5 Protein (His tag)

Catalog No. ABIN7195471

Product Details

Target: ENTPD5 (Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This ENTPD5 protein is labelled with His tag.

Purpose: Recombinant Human ENTPD5 Protein (His Tag)(Active)

Sequence: Met 1-His 428

Characteristics: A DNA sequence encoding the human ENTPD5 (O75356) (Met 1-His 428) was fused with a polyhistidine tag at the C-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to hydrolyze the 5'phosphate groups from the substrate guanosine5'diphosphate (GDP).The specific activity is >7,000 pmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ENTPD5 (Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5))

Alternative Name: ENTPD5 (ENTPD5 Products)

Synonyms: ENTPD5 Protein, CD39L4 Protein, NTPDase-5 Protein, PCPH Protein, AI196558 Protein, AI987697 Protein, Cd39l4 Protein, ER-UDPase Protein, NTPDase5 Protein, Pcph Protein, mNTPase Protein, cph Protein, ectonucleoside triphosphate diphosphohydrolase 5 Protein, ectonucleoside triphosphate diphosphohydrolase 5 L homeolog Protein, ENTPD5 Protein, entpd5 Protein, entpd5.L Protein, Entpd5 Protein

Background:

Background: Ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5), also known as CD39 antigen-like 4, ER-UDPase, Guanosine-diphosphatase ENTPD5, Nucleoside diphosphatase Uridine-diphosphatase ENTPD5. This hydrolase is expressed in response to phosphoinositide 3-kinase (PI3K) signaling. Activation of PI3K results in FOXO phosphorylation by AKT1 and loss of ENTPD5 transcriptional repression. It is Up-regulated in PTEN-deficient cells. Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation.ENTPD5 promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, ENTPD5 constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. ENTPD5 also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. This enzyme Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.

Synonym: CD39L4,NTPDase-5,PCPH

Molecular Weight: 47 kDa

UniProt: O75356