EPH Receptor A2 Protein (EPHA2) (AA 585-976) (His-GST)

Catalog No. ABIN7317048

This Recombinant EPH Receptor A2 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for EPH Receptor A2 Protein (EPHA2) (AA 585-976) (His-GST) (ABIN7317048)

Target: EPH Receptor A2 (EPHA2)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 585-976

Purification tag / Conjugate: This EPH Receptor A2 protein is labelled with His-GST.

Purpose: Recombinant Human EphA2 Protein (aa 585-976, His & GST Tag)

Sequence: Leu 585-Ile 976

Characteristics: A DNA sequence encoding the human EPHA2 (P29317) (Leu585-Ile976) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 50 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 3 mM DTT, pH 8.5, 10 % glycerol

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: EPH Receptor A2 (EPHA2)

Alternative Name: EphA2

Background: ARCC2,CTPA,CTPP1,CTRCT6,ECK,EPHA2,Ephrin type-A receptor 2,Epithelial cell kinase,Tyrosine-protein kinase receptor ECK,Eph receptor A2 is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. The Eph receptors' corresponding family of ligands are the ephrins anchored to cell surfaces. The ephrins and Eph receptors are implicated as positional labels that may guide the development of neural topographic maps. They have also been found implicated in embryonic patterning, neuronal targeting, vascular development and adult neovascularization. The large family of ligands and receptors may make a major contribution to the accurate spatial patterning of connections and cell position in the nervous system. Furthermore, elevated expression of Eph receptors and ephrin ligands is associated with tumors and associated tumor vasculature, suggesting the Eph receptors and ephrin ligands also play critical roles in tumor angiogenesis and tumor growth. Unlike most Eph kinases, which are primarily expressed during development, EphA2 is primarily found in adult human epithelial cells. The cellular functions of EphA2 may be regulating cell growth, survival, migration, and angiogenesis.Unlike other receptor tyrosine kinases, ligand binding is not necessary for EphA2. Rather, the ligand appears to regulate EphA2 subcellular localization and its interactions with downstream adapter and signaling proteins. Eph receptor A2(EphA2) has been demonstrated to critically regulate tumor cell growth, migration and invasiveness. Eph receptor A2(EphA2) is frequently overexpressed and functionally altered in aggressive tumor cells, and that these changes promote metastatic character.

Molecular Weight:

Calculated MW: 72.1 kDa

Observed MW: 62 kDa

Gene ID: 1969

UniProt: P29317

Pathways: RTK Signaling