EPH Receptor A4 Protein (EPHA4) (AA 570-986) (His-GST)

Catalog No. ABIN7317076

This Recombinant EPH Receptor A4 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for EPH Receptor A4 Protein (EPHA4) (AA 570-986) (His-GST) (ABIN7317076)

Target: EPH Receptor A4 (EPHA4)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Application: ELISA

Purity: > 99 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 570-986

Purification tag / Conjugate: This EPH Receptor A4 protein is labelled with His-GST.

Purpose: Recombinant Human EphA4 Protein (aa 570-986, His &GST Tag)

Sequence: Ser 570-Val 986

Characteristics: A DNA sequence encoding the human EPHA4 (P54764) (Ser570-Val986) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. The specific activity was determined to be 17 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate. 2. Immobilized human EPHA4 (aa 570-986)at 10 μg/ml (100 μl/well) can bind biotinylated human EphrinA5-His with a linear range of 0.625-5.0 μg/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.5, 10 % glycerol, 3 mM DTT

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: EPH Receptor A4 (EPHA4)

Alternative Name: EphA4

Background: EK8;EPH-like kinase 8;EPHA4;Ephrin type-A receptor 4;HEK8;SEK;TYRO1;Tyrosine-protein kinase TYRO1;Tyrosine-protein kinase receptor SEK;hEK8,EPH receptor A4 (ephrin type-A receptor 4); also known as EphA4; belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1; EPHA2; EPHA3; EPHA4; EPHA5; EPHA6; EPHA7; EPHA8; EPHA9; EPHA10; EPHB1; EPHB2; EPHB3; EPHB4; EPHB5; EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. EphA4 is enriched on dendritic spines of pyramidal neurons in the adult mouse hippocampus; and ephrin-A3 is localized on astrocytic processes that envelop spines. Eph receptor−mediated signaling; which is triggered by ephrins7; probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development; forming the largest sub-family of receptor tyrosine kinases (RTKs). The extracellular domain of an EphA4 interacts with ephrin ligands; which may be tethered to neighbouring cells. Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer.

Molecular Weight:

Calculated MW: 75.0 kDa

Observed MW: 67 kDa

Gene ID: 2043

UniProt: P54764

Pathways: RTK Signaling