EPH Receptor A7 Protein (EPHA7) (AA 579-998) (His-GST)

Catalog No. ABIN7317067

Recombinant EPH Receptor A7 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for EPH Receptor A7 Protein (EPHA7) (AA 579-998) (His-GST) (ABIN7317067)

Target: EPH Receptor A7 (EPHA7)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 94 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 579-998

Purification tag / Conjugate: This EPH Receptor A7 protein is labelled with His-GST.

Purpose: Recombinant Human EphA7/EHK3 Protein (His & GST Tag)

Sequence: Gly579-Val998

Characteristics: A DNA sequence encoding the human EPHA7 (NP_004431) (Gly579-Val998) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 9.5 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: EPH Receptor A7 (EPHA7)

Alternative Name: EphA7/EHK3

Background: EHK-3,EHK3,EK11,EPH Homology Kinase 3,EPH-Like Kinase 11,EPHA7,Ephrin Type-A Receptor 7,HEK11,hEK11,Ephrin type-A receptor 7, also known as EphA7, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Eph receptor-mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. Down-regulation of EphA7 secondary to hypermethylation has been reported in colorectal cancer. The expression of EphA7 was reduced in all tested gastric cancer cell lines, however, there is marked variability in expression among gastric carcinoma specimens. EphA7 may have roles in the pathogenesis and development of gastric carcinomas.

Molecular Weight:

Calculated MW: 75.2 kDa

Observed MW: 76 kDa

Gene ID: 2045

NCBI Accession: NP_004431

UniProt: Q15375

Pathways: RTK Signaling