EPH Receptor B2 Protein (EPHB2) (AA 570-987) (GST tag,His tag)

Catalog No. ABIN7317086

Recombinant EPH Receptor B2 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for EPH Receptor B2 Protein (EPHB2) (AA 570-987) (GST tag,His tag) (ABIN7317086)

Target: EPH Receptor B2 (EPHB2)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 570-987

Purification tag / Conjugate: This EPH Receptor B2 protein is labelled with GST tag,His tag.

Purpose: Recombinant Human EphB2 Protein (aa 570-987, His &GST Tag)(Active)

Sequence: Gly570-Val987

Characteristics: A DNA sequence encoding the human EPHB2 (P29323-3) (Gly570-Val987) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 120 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 3 mM DTT, 10 % glycerol

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: EPH Receptor B2 (EPHB2)

Alternative Name: EphB2

Background:

Background: Ephrin type-B receptor 2, also known as EphB2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Synonym: CAPB,DRT,EK5,EPHT3,ERK,Hek5,PCBC,Tyro5,EPHB2,Ephrin type-B receptor 2

Molecular Weight: 75.2 kDa

Pathways: RTK Signaling, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins