Ephrin A5 Protein (EFNA5) (His tag)

Catalog No. ABIN7320515

Recombinant Ephrin A5 protein expressed in Human Cells.

Quick Overview for Ephrin A5 Protein (EFNA5) (His tag) (ABIN7320515)

Target: Ephrin A5 (EFNA5)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Human Cells

Purity: > 90 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This Ephrin A5 protein is labelled with His tag.

Purpose: Recombinant Mouse Ephrin-A5/EFNA5 Protein (His Tag)(Active)

Sequence: Gln21-Gln206

Characteristics: Recombinant Mouse Ephrin-A5 is produced by our Mammalian expression system and the target gene encoding Gln21-Gln206 is expressed with a 6His tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human EphA8-Fc(Cat: PKSH032386) at 1.5μg/ml(100 μl/well) can bind Human EFNA5-His. The ED50 of EFNA5-His is 12.63ug/ml .

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Ephrin A5 (EFNA5)

Alternative Name: Ephrin-A5/EFNA5

Background:

Background: Ephrin-A5 is a glycosylphosphatidylinositol (GPI)-anchored protein of the ephrin-A subclass of ephrin ligands that binds to the EphA subclass of Eph receptors. Ephrin-A5 has also been shown to bind to the EphB2 receptor. It is crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Ephrin-A5 binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.

Synonym: Ephrin-A5, AL-1, EPH-related receptor tyrosine kinase ligand 7, Epl7, Eplg7, Lerk7, Efna5,

Molecular Weight: 22.5 kDa

UniProt: O08543

Pathways: RTK Signaling