FGF1 Protein (AA 16-155) (His tag)

Catalog No. ABIN7318475

This Recombinant FGF1 protein is produced in Escherichia coli (E. coli).

Quick Overview for FGF1 Protein (AA 16-155) (His tag) (ABIN7318475)

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Purity: > 98 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 16-155

Purification tag / Conjugate: This FGF1 protein is labelled with His tag.

Purpose: Recombinant Human FGF-1/FGFa Protein

Sequence: Phe16-Asp155

Characteristics: Recombinant Human Fibroblast growth factor 1/Fibroblast Growth Factor Acidic is produced by our E.coli expression system and the target gene encoding Phe16-Asp155 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <0.3 ng/mL. The specific activity of recombinant human FGF-1 is > 1 x 10⁶ IU/mg.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Alternative Name: FGF-1/FGFa

Background: Acidic Fibroblast Growth Factor,ECGFHeparin-Binding Growth Factor 1,Endothelial Cell Growth Factor,FGF-1,FGF1,FGFA,Fibroblast Growth Factor 1,HBGF-1,aFGF,FGF acidic, also known as ECGF, FGF-1and HBGF-1, is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. It is a mitogenic peptide that is produced by multiple cell types and stimulates the proliferation of cells of mesodermal, ectodermal, and endodermal origin. Its association with heparan sulfate is a prerequisite for activation of FGF receptors. Internalized FGF acidic migrates to the nucleus where it is phosphorylated by nuclear PKC delta, exported to the cytosol, dephosphorylated, and degraded. Intracellular FGF acidic inhibits p53 activity and proapoptotic signaling.

Molecular Weight:

Calculated MW: 16.8 kDa

Observed MW: 18 kDa

Gene ID: 2246

UniProt: P05230

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway