GZMB Protein (His tag)

Catalog No. ABIN7195987

Product Details

Target: GZMB (Granzyme B (GZMB))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This GZMB protein is labelled with His tag.

Purpose: Recombinant Human Granzyme B/GZMB Protein (His Tag)

Sequence: Met 1-Tyr 247

Characteristics: A DNA sequence encoding the proform of human Granzyme B (NP_004122.1) (Met 1-Tyr 247) was expressed with a C-terminal polyhistidine tag.

Purity: > 97 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: GZMB (Granzyme B (GZMB))

Alternative Name: Granzyme B/GZMB (GZMB Products)

Synonyms: CCPI Protein, CGL-1 Protein, CGL1 Protein, CSP-B Protein, CSPB Protein, CTLA1 Protein, CTSGL1 Protein, HLP Protein, SECT Protein, granzyme B Protein, GZMB Protein

Background:

Background: Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 30 substrates of GZMB have been identified including the key substrate caspase-3, ICAD and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells, and accordingly plays an essential role in immunosurveillance.

Synonym: CCPI,CGL-1,CGL1,CSP-B,CSPB,CTLA1,CTSGL1,HLP,SECT

Molecular Weight: 27 kDa

NCBI Accession: NP_004122

Pathways: Apoptosis, Caspase Cascade in Apoptosis