HK3 Protein (His-GST)
Quick Overview for HK3 Protein (His-GST) (ABIN7196087)
Target
See all HK3 ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This HK3 protein is labelled with His-GST.
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Purpose
- Recombinant Human Hexokinase-3/HK3 Protein (His & GST Tag)
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Sequence
- Met 1-Val 923
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Characteristics
- A DNA sequence encoding the human HK3 (P52790) (Met 1-Val 923) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Hexokinase 3 (White Cell) (HK3) (AA 1-923) protein (GST tag)
HK3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Hexokinase 3 (White Cell) (HK3) (AA 1-61) protein (His-IF2DI Tag)HK3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Hexokinase 3 (White Cell) (HK3) protein (Myc-DYKDDDDK Tag)HK3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as sterile solution of 50 mM Tris, 100 mM NaCl, pH 7.4
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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Expiry Date
- 6 months
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- HK3 (Hexokinase 3 (White Cell) (HK3))
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Alternative Name
- Hexokinase-3/HK3
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Background
- HKIII,HXK3,Hexokinase-3, also known as Hexokinase type III, HKIII and HK3, is a protein which belongs to thehexokinase family. Hexokinase-3 / HK3 is anenzymewhich in humans is encoded by theHK2gene. Hexokinases phosphorylate glucoseto produceglucose 6-phosphate, committing glucose to theglycolytic pathway. In mammalian tissues hexokinase exists as four isoenzymes encoded by distinct genes. These proteins are homologous and are organized in two homologous domains, with the exception of hexokinase type IV which has only one. This organization is believed to be the result of a duplication and tandem fusion event involving the gene encoding for the ancestral hexokinase. The gene encodes hexokinase-3. Similar to hexokinases-1 and hexokinases-2, thisallostericenzyme is inhibited by its product glucose 6-phosphate.
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Molecular Weight
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Calculated MW: 127 kDa
Observed MW: 115 kDa
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Gene ID
- 3101
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UniProt
- P52790
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Pathways
- Carbohydrate Homeostasis, Warburg Effect
Target
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