ICAM2 Protein

Catalog No. ABIN7196247

This Recombinant ICAM2 protein is produced in HEK-293 Cells.

Quick Overview for ICAM2 Protein (ABIN7196247)

Target: ICAM2 (Intercellular Adhesion Molecule 2 (ICAM2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 85 % as determined by SDS-PAGE

Product Details

Purpose: Recombinant Mouse ICAM-2/CD102 Protein (Active)

Sequence: Met 1-Gln 222

Characteristics: A DNA sequence encoding the mouse ICAM2 (NP_034624.1) (Met 1-Gln 222)was expressed with six amino acids (LEVLFQ) at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.When 5 x 10E4 cells/well are added to rmICAM1 coated plates (12.5 μg/mL with 100 μL/well), > 30% cells will adhere after 1 hour incubation at 37°C.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ICAM2 (Intercellular Adhesion Molecule 2 (ICAM2))

Alternative Name: ICAM-2/CD102

Background:

Background: Intercellular adhesion molecule 2 (ICAM-2, CD102), belongs to the ICAM family consisting of three members identified as ligands for integrin receptors. It is a type I transmembrane glycoprotein with two Ig-like C2-type domains, and binds to the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). As a second ligand of leukocyte function-associated antigen-1, ICAM-2 functions as a costimulatory molecule for effector cells. ICAM-2 is mainly expressed on vascular endothelial and hematopoietic cells. Interactions of ICAM-2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocytewith other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity and migration, lymphocyte recirculation, etc. Serum levels of ICAM-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relation with blood eosinophilia. So, estimation of ICAM-2 serum levels may prove useful in diagnosis of trichinosis recent infections, and in monitoring the prognosis and response to treatment.

Synonym: AILIM Protein, CCLP Protein, CRP-1 Protein, H4 Protein, Ly115 Protein

Molecular Weight: 23.6 kDa

NCBI Accession: NP_034624