IL-2 Protein (AA 21-169)

Catalog No. ABIN7320817

Recombinant IL-2 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-2 Protein (AA 21-169) (ABIN7320817)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 21-169

Purpose: Recombinant Mouse Interleukin-2/IL-2 Protein

Sequence: Ala21-Gln169

Characteristics: Recombinant Mouse Interleukin-2 is produced by our E.coli expression system and the target gene encoding Ala21-Gln169 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.01 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells.The specific activity of Recombinant Mouse IL-2 is ≥1x10⁷ IU/mg.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Sodium Citrate, 0.2 % Tween 80, pH 3.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: Interleukin-2/IL-2

Background: POIL,OTTHUMP,Aldesleukin,IL 2,Interleukin 2,Involved in regulation of T cell clonal expansion,Lymphokine,OTTHUMP00000164090,POIL2,T Cell Growth Factor,T-cell growth facter,Interleukin-2,IL2,TCGF,T-cell growth factor,Il-2,Interleukin 2 (IL 2), also termed T-cell growth factor, is a member of the cytokine family which includes IL-4, IL-7, IL-9, IL-15 and IL-21. Each member of this family has a four alpha helix bundle. IL-2 signals through the IL-2 receptor, a complex consisting of tree subunits, termed alpha, beta and gamma. The IL-2 R gamma is shared by cytokine receptors of all members of cytokine family. Mature mouse IL-2 shares 56 % and 73 % aa sequence identity with human and rat IL-2, respectively. IL-2 is produced by CD4+ T cell, CD8+ T cells, gamma δ T cells, B cells, dendritic cells and eosinophils, and plays a vital role in key function of the immune system, tolerance and immunity, primarily via its potent stimulatory activity for T cells.Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity.

Molecular Weight:

Calculated MW: 17.4 kDa

Observed MW: 17 kDa

Gene ID: 16183

UniProt: P04351

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation