IL-4 Protein (AA 25-153)

Catalog No. ABIN7319404

This Recombinant IL-4 protein is expressed in HEK-293 Cells.

Quick Overview for IL-4 Protein (AA 25-153) (ABIN7319404)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Cell Culture (CC)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 25-153

Purpose: Recombinant Human Interleukin-4/IL-4 Protein

Sequence: His25-Ser153

Characteristics: Recombinant Human Interleukin-4 is produced by our Mammalian expression system and the target gene encoding His25-Ser153 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.01 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.01-0.05 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: Interleukin-4/IL-4

Background: Interleukin,B-cell growth factor,BSF,IL4,BCGF-1,BCGF1,BSF-1,BSF1,IL-4,B-cell growth factor 1,B-cell IgG differentiation factor,B-cell stimulatory factor 1,Binetrakin,IGG1 induction factor,Interleukin-4,Lymphocyte stimulatory factor 1,Pitrakinra,Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.

Molecular Weight:

Calculated MW: 15.0 kDa

Observed MW: 18 kDa

Gene ID: 3565

UniProt: P05112

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation