IL4 Receptor Protein (His tag)

Catalog No. ABIN7196569

This Recombinant IL4 Receptor protein is expressed in HEK-293 Cells.

Quick Overview for IL4 Receptor Protein (His tag) (ABIN7196569)

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293 Cells

Purity: > 95 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This IL4 Receptor protein is labelled with His tag.

Purpose: Recombinant Rat IL4RA/CD124 Protein (His Tag)(Active)

Sequence: Met1-Arg232

Characteristics: A DNA sequence encoding the rat IL4R (Q63257-1) (Met1-Arg232) was expressed, fused with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Measured by its ability to bind biotinylated mouse IL4-His in a functional ELISA.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Alternative Name: IL4RA/CD124

Background:

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD124, also known as interleukin 4 receptor (IL4R), is a typeI transmembrane protein that can regulate IgE antibody production in B cells through binding to interleukin 4 and interleukin 13 and promote differentiation of Th2 cells through binding to interleukin 4. The membrane-bound form of CD124 can be hydrolyzed to soluble form which can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells.

Synonym: IL4R,Il4ra

Molecular Weight: 25.3 kDa

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process