KEAP1 Protein
Quick Overview for KEAP1 Protein (ABIN7317443)
Target
See all KEAP1 ProteinsProtein Type
Origin
Source
Purity
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Purpose
- Recombinant Human KEAP1/INRF2 Protein
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Sequence
- Gln2-Cys624
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Characteristics
- A DNA sequence encoding the human KEAP1 (Q14145) (Gln2-Cys624) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Kelch-Like ECH-Associated Protein 1 (KEAP1) (AA 1-624) protein (His tag)
custom-made KEAP1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Kelch-Like ECH-Associated Protein 1 (KEAP1) (AA 1-624) protein (His tag)custom-made KEAP1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Kelch-Like ECH-Associated Protein 1 (KEAP1) (AA 1-624) protein (GST tag)KEAP1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Kelch-Like ECH-Associated Protein 1 (KEAP1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)KEAP1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Kelch-Like ECH-Associated Protein 1 (KEAP1) (AA 378-486) protein (His-IF2DI Tag)KEAP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please refer to the printed manual for detailed information.
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Buffer
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 3 mM DTT, 10 % glycerol, pH 7.4
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))
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Alternative Name
- KEAP1/INRF2
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Background
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Background: Kelch-like ECH-associated protein 1, also known as cytosolic inhibitor of Nrf2, Kelch-like protein 19, KEAP1 and INRF2, is a cytoplasm and nucleus protein which contains one BACK (BTB/Kelch associated) domain, one BTB (POZ) domain and six Kelch repeats. KEAP1 / INRF2 is broadly expressed, with highest levels in skeletal muscle. KEAP1 / INRF2 is a key regulator of the NRF2 transcription factor, which transactivates the antioxidant response element (ARE) and upregulates numerous proteins involved in antioxidant defense. Under basal conditions, KEAP1 / INRF2 targets NRF2 for ubiquitination and proteolytic degradation and as such is responsible for the rapid turnover of NRF2. KEAP1 / INRF2 retains NFE2L2 / NRF2 in the cytosol. KEAP1 / INRF2 functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. It targets NFE2L2 / NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. KEAP1 / INRF2 may also retain BPTF in the cytosol. It targets PGAM5 for ubiquitination and degradation by the proteasome.
Synonym: INRF2,KEAP-1,KLHL19
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Molecular Weight
- 69.7 kDa
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UniProt
- Q14145
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Pathways
- Maintenance of Protein Location
Target
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