Lacritin Protein (LACRT) (His tag)
-
- Target See all Lacritin (LACRT) products
- Lacritin (LACRT)
- Protein Type
- Recombinant
- Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Lacritin protein is labelled with His tag.
- Purpose
- Recombinant Human Lacritin/LACRT Protein (His Tag)
- Sequence
- Ala19-Ala138
- Characteristics
- Recombinant Human Lacritin is produced by our E.coli expression system and the target gene encoding Ala19-Ala138 is expressed with a 6His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
-
Lacritin (LACRT) (AA 20-138) protein (His tag)
LACRT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Lacritin (LACRT) protein (Myc-DYKDDDDK Tag)LACRT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- Target
- Lacritin (LACRT)
- Alternative Name
- Lacritin/LACRT (LACRT Products)
- Synonyms
- lacritin Protein, LACRT Protein
- Background
-
Background: Extracellular glycoprotein lacritin (Lacritin) is a secreted protein which consists of 119 amino acids after cleavage of the N-terminal signal peptide and displays several predicted alpha helices, mostly in the C-terminal half. Lacritin is highly expressed in the lacrimal gland, localizes primarily to secretory granules and secretory fluid. Lacritin modulates lacrimal acinar cell secretion, promotes ductal cell proliferation, and stimulates signaling through tyrosine phosphorylation and release of calcium. Lacritin is thus a multifunctional prosecretory mitogen with cell survival activity. Natural or bacterial cleavage of lacritin releases a C-terminal fragment that is bactericidal. Lacritin cell targeting is dependent on the cell surface heparan sulfate proteoglycan syndecan-1 (SDC1). Binding utilizes an enzyme-regulated 'off-on' switch in which active epithelial heparanase (HPSE) cleaves off heparan sulfate to expose a binding site in the N-terminal region of syndecan-1's core protein.
Synonym: Extracellular Glycoprotein Lacritin, LACRT
- Molecular Weight
- 14.6 kDa
- UniProt
- Q9GZZ8
-
