LIF Protein

Catalog No. ABIN7565330

This Recombinant LIF protein is produced in HEK-293 Cells.

Quick Overview for LIF Protein (ABIN7565330)

Target: LIF (Leukemia Inhibitory Factor (LIF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Cell Culture (CC)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human LIF Protein (HEK293 Cells)

Sequence: Met 1-Phe202

Characteristics: A DNA sequence encoding the human LIF (P15018) (Met1-Phe202) was expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED₅₀ for this effect is typically 0.2-0.8 ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: LIF (Leukemia Inhibitory Factor (LIF))

Alternative Name: LIF

Background: CDF;D Factor;DIA;Differentiation-Stimulating Factor;Emfilermin;HILDA;LIF;Leukemia Inhibitory Factor;MLPLI;Melanoma-Derived LPL Inhibitor,Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It’s involved in growth promotion and cell differentiation of different types of target cells; influence on bone metabolism; cachexia; neural development; embryogenesis and inflammation. LIF has potent proinflammatory property; being the inducer of the acute phase protein synthesis and affecting the cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells; hematopoietic and neuronal cells. LIF binds to the specific LIF receptor (LIFR-α) which forms a heterodimer with a specific subunit common to all members of that family of receptors; the GP130 signal transducing subunit. This leads to activation of the JAK/STAT and MAPK cascades. Due to its polyfunctional activities; LIF is involved in the pathogenic events and development of many diseases of various origin.

Molecular Weight:

Calculated MW: 19.7 kDa

Observed MW: 35.4 kDa

Gene ID: 3976

UniProt: P15018

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding