LAIR2 Protein

Catalog No. ABIN7317737

This Recombinant LAIR2 protein is produced in HEK-293 Cells.

Quick Overview for LAIR2 Protein (ABIN7317737)

Target: LAIR2 (Leukocyte-Associated Immunoglobulin-Like Receptor 2 (LAIR2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 93 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human LAIR2/CD306 Protein (Active)

Sequence: Met 1-Pro 152

Characteristics: A DNA sequence encoding the human LAIR2 (NP_002279.2) (Met 1-Pro 152) was expressed and purified.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of HT-29 human colon adenocarcinoma cells. When 5 x 10E4 cells/well are added to recombiannt human LAIR2 coated plates (50 μg/ml with 100 μl/well), >30% will adhere after 30 minutes at 37°C. Optimal concentration depends on cell type as well as the application or research objectives.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 0.7M NaCl, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: LAIR2 (Leukocyte-Associated Immunoglobulin-Like Receptor 2 (LAIR2))

Alternative Name: LAIR2/CD306

Background:

Background: Leukocyte-associated immunoglobulin-like receptor 2 ( LAIR2 ), also known as CD306, is a 131 amino acid protein containing one lg-like C2-type domain. It is expressed as a soluble receptor exhibiting high affinity for various collagen molecules to which it binds in a hydroxyproline-dependent manner. LAIR2 is a member of the immunoglobulin superfamily and was identified by its similarity to LAIR1, an inhibitory receptor present on mononuclear leukocytes. LAIR2 is thought to be secreted and may help modulate mucosal tolerance. As a natural competitor for LAIR1, soluble LAIR2 prevents binding of human LAIR1 to collagens and LAIR1 cross-linking, thereby regulating its inhibitory potential. Accordingly, LAIR2 is suggested to perform an immunoregulatory function.

Synonym: Leukocyte-Associated Immunoglobulin-Like Receptor 2, LAIR-2, CD306, LAIR2

Molecular Weight: 14.1 kDa

NCBI Accession: NP_002279