LAIR2 Protein (AA 22-152) (His tag,AVI tag,Biotin)

Catalog No. ABIN5674601

Product Details

Target: LAIR2 (Leukocyte-Associated Immunoglobulin-Like Receptor 2 (LAIR2))

Protein Type: Recombinant

Protein Characteristics: AA 22-152

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This LAIR2 protein is labelled with His tag,AVI tag,Biotin.

Brand: PrecisionAvi

Sequence: AA 22-152

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 17.8 kDa. The protein migrates as 25-28 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >95 % as determined by reduced SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: LAIR2 (Leukocyte-Associated Immunoglobulin-Like Receptor 2 (LAIR2))

Alternative Name: LAIR-2 (LAIR2 Products)

Synonyms: CD306 Protein, leukocyte associated immunoglobulin like receptor 2 Protein, LAIR2 Protein

Background: Leukocyte-associated immunoglobulin-like receptor 2 (LAIR2) is also known as CD antigen CD306. LAIR2 contains one Ig-like C2-type (immunoglobulin-like) domain. LAIR2 is a member of the immunoglobulin superfamily and was identified by its similarity to LAIR1, an inhibitory receptor present on mononuclear leukocytes. LAIR2 / CD306 is thought to be secreted and may help modulate mucosal tolerance.

Molecular Weight: 17.8 kDa

NCBI Accession: NP_002279