METTL11A Protein

Catalog No. ABIN7317612

This Recombinant METTL11A protein is produced in Escherichia coli (E. coli).

Quick Overview for METTL11A Protein (ABIN7317612)

Target: METTL11A (Methyltransferase Like 11A (METTL11A))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human METTL11A Protein

Sequence: Thr 2-Arg 223

Characteristics: A DNA sequence encoding the human METTL11A (NP_054783.2) (Thr 2-Arg 223) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.

Sterility: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 150 mM NaCl, 10 % glycerol, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: METTL11A (Methyltransferase Like 11A (METTL11A))

Alternative Name: METTL11A

Background: AD-003,C9orf32,HOMT1A,METTL11A,NRMT,NTM1A,Methyltransferase-like protein 11A, also known as METTL11A, is a member of the methyltransferase superfamily and METTL11 family. Methyltransferase is a type of transferase enzyme which transfers a methyl group from a donor to an acceptor. Methylation often occurs on nucleic bases in DNA or amino acids in protein structures. Methytransferase uses a reactive methyl group bound to sulfur in S-adenosyl methionine (SAM) as the methyl donor. DNA methylation is often utilized to silence and regulate genes without changing the original DNA sequence. This methylation occurs on cytosine residues. DNA methylation may be necessary for normal growth from embryonic stages in mammals. Methylation can serve to protect DNA from enzymatic cleavage, since restriction enzymes are unable to bind and recognize externally modified sequences. This is especially useful in bacterial restriction modification systems which use restriction enzymes to cleave foreign DNA while keeping their own DNA protected by methylation. Methylation of amino acids in the formation of proteins leads to more diversity of possible amino acids and therefore more diversity of function. The methylation reaction occurs on nitrogen atoms either on the N terminus or side-chain position of the protein and are usually irreversible.

Molecular Weight:

Calculated MW: 25.5 kDa

Observed MW: 25.5 kDa

Gene ID: 28989

NCBI Accession: NP_054783

UniProt: Q9BV86