MAPKAP Kinase 5 Protein (His-GST)
Quick Overview for MAPKAP Kinase 5 Protein (His-GST) (ABIN7317049)
Target
See all MAPKAP Kinase 5 (MAPKAPK5) ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This MAPKAP Kinase 5 protein is labelled with His-GST.
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Purpose
- Recombinant Human MAPKAPK5 Protein (His & GST Tag)
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Sequence
- Met 1-Gln 471
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Characteristics
- A DNA sequence encoding the human MAPKAPK5 isoform 1 (NP_003659.2) (Met 1-Gln 471) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (AA 1-473) protein (His tag)
custom-made MAPKAPK5 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (AA 1-473) protein (His tag)custom-made MAPKAPK5 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (AA 1-471) protein (GST tag)MAPKAPK5 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA, KAA
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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Expiry Date
- 6 months
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- MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
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Alternative Name
- MAPKAPK5
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Background
- MAPKAP-K5,MK-5,MK5,PRAK,MAPKAPK5 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family. MAPKAPK5 has significant sequence homology to mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK). It is widely distributed. MAPKAPK5 can be phosphorylated by extracellular-regulated kinase (ERK), and p38 kinase but not by c-jun N-terminal kinase (JNK)in vitro.Recombinant GST-MAPKAPK5 protein can phosphorylate a peptide derived from the regulatory light chain of myosin II. Phosphorylation of MAPKAPK5 by ERK and p38 kinase increased its activity by 9 and 15 fold respectively. Taken together, these data suggest that MAPKAPK5 is a novelin vitrosubstrate for ERK and p38 kinase. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. MAPKAPK5 also mediates stress-induced small heat shock protein 27 phosphorylation.
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Molecular Weight
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Calculated MW: 82.0 kDa
Observed MW: 75 kDa
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Gene ID
- 8550
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NCBI Accession
- NP_003659
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UniProt
- Q8IW41
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Pathways
- MAPK Signaling
Target
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