Nemo-Like Kinase Protein (NLK) (His-GST)

Catalog No. ABIN7317069

This Recombinant Nemo-Like Kinase protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for Nemo-Like Kinase Protein (NLK) (His-GST) (ABIN7317069)

Target: Nemo-Like Kinase (NLK)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 91 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Nemo-Like Kinase protein is labelled with His-GST.

Purpose: Recombinant Human NLK Protein (His & GST Tag)

Sequence: Val121-Glu 527

Characteristics: A DNA sequence encoding the human NLK (Q9UBE8) (Val121-Glu527) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 3 nmol/min/mg using MBP as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: Nemo-Like Kinase (NLK)

Alternative Name: NLK

Background: LAK1,NLK,Nemo-like kinase contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and MAP kinase subfamily. It also contains a TQE activation loop motif in which autophosphorylation of the threonine residue (Thr-298) is sufficient for kinase activation. As a serine/threonine-protein kinase, nemo-like kinase regulates a number of transcription factors with key roles in cell fate determination. It is a positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Nemo-like kinase also is a negative regulator of the canonical Wnt/beta-catenin signaling pathway.

Molecular Weight:

Calculated MW: 74.1 kDa

Observed MW: 73 kDa

Gene ID: 51701

UniProt: Q9UBE8

Pathways: Ubiquitin Proteasome Pathway