Nemo-Like Kinase Protein (NLK)

Catalog No. ABIN7939365

This Recombinant Nemo-Like Kinase protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for Nemo-Like Kinase Protein (NLK) (ABIN7939365)

Target: Nemo-Like Kinase (NLK)

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Purpose: Recombinant NLK protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant NLK protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: Nemo-Like Kinase (NLK)

Alternative Name: NLK

Background:

Protein Background: NLK (Nemo Like Kinase), a serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2 and negative regulator of the canonical Wnt/beta-catenin signaling pathway. NLK binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. NLK acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Diseases associated with NLK include Diamond-Blackfan Anemia.

Short Description: Recombinant NLK protein was expressed in baculovirus expression system as the full length protein (accession number NP_057315.3) with a N-terminal FLAG tag. The molecular weight of the protein is 59.5 kDa.

Aliases: Nemo Like Kinase

Pathways: Ubiquitin Proteasome Pathway