NPTN Protein (AA 29-220) (His tag)

Catalog No. ABIN7318820

Recombinant NPTN protein expressed in HEK-293 Cells.

Quick Overview for NPTN Protein (AA 29-220) (His tag) (ABIN7318820)

Target: NPTN (Neuroplastin (NPTN))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 29-220

Purification tag / Conjugate: This NPTN protein is labelled with His tag.

Purpose: Recombinant Human Neuroplastin/NPTN Protein (His Tag)

Sequence: Gln29-His220

Characteristics: Recombinant Human Neuroplastin is produced by our Mammalian expression system and the target gene encoding Gln29-His220 is expressed with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: NPTN (Neuroplastin (NPTN))

Alternative Name: Neuroplastin/NPTN

Background: NPTN,Neuroplastin,SDFR1,SDR-1,SDR1,Stromal Cell-Derived Receptor 1,Neuroplastin (NPTN) is a 52-57 kDa member of the Ig-superfamily. Neuroplastin likely serves as a cell adhesion molecule, and is widely expressed in multiple tissues. Human Neuroplastin is 282 amino acids that contions two Ig-like domains and a 38 aa cytoplasmic region. Probable homophilic and heterophilic cell adhesion molecule involved in long term potentiation at hippocampal excitatory synapses through activation of p38MAPK. Neuroplastin may also regulate neurite outgrowth by activating the FGFR1 signaling pathway. It may play a role in synaptic plasticity.

Molecular Weight:

Calculated MW: 22.7 kDa

Observed MW: 35-55 kDa

Gene ID: 27020

UniProt: Q9Y639

Pathways: Regulation of long-term Neuronal Synaptic Plasticity