Notch1 Protein (His tag)

Catalog No. ABIN7197217

This Recombinant Notch1 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for Notch1 Protein (His tag) (ABIN7197217)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Baculovirus infected Insect Cells

Application: ELISA

Purity: > 82 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Notch1 protein is labelled with His tag.

Purpose: Recombinant Mouse Motch A/NOTCH1 Protein (His Tag)

Sequence: Met 1-Gln 526

Characteristics: A DNA sequence encoding the mouse NOTCH1 (NP_032740.3) N-terminal fragment (Met 1-Gln 526) was expressed,with a C-terminal polyhistidine tag.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. Immobilized human DLL4 at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC₅₀ of biotinylated mouse NOTCH1-his is 40 ng/mL. 2. Immobilized mouse DLL4-his at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC₅₀ of biotinylated mouse NOTCH1-his is 30 ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: Motch A/NOTCH1

Background: Mis6,Motch A,Mt14,N1,Neurogenic locus notch homolog protein 1,Notch 1,Tan1,lin-12,NOTCH1 is one of the four mammalian Notch receptors, which is involved in the Notch signaling pathway. Specifically, NOTCH1 promotes the proliferation of myogenic precursor cells, and the NICD domain of NOTCH1 can impair regeneration of skeletal muscles.NOTCH1 is a prevalent signaling pathway in T cell acute lymphoblastic leukemia (T-ALL).The NOTCH signaling pathway is a conserved signaling cascade that regulates many aspects of development and homeostasis in multiple organ systems.The proto-oncogene NOTCH1 is frequently mutated in around 1 % of patients with chronic lymphocytic leukemia (CLL). NOTCH1 mutations in oral squamous cell carcinoma (OSCC) frequently occur near the ligand-binding region. These mutations change the domain structure of this protein and affect the ligand binding activity. When NOTCH1 is activated by ligand binding, NOTCH1 intracellular domain (NICD) is cleaved from the cell membrane.

Molecular Weight:

Calculated MW: 55.0 kDa

Observed MW: 80 kDa

NCBI Accession: NP_032740

UniProt: Q01705

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development