PDE1C Protein (His-GST)

Catalog No. ABIN7197331

This Recombinant PDE1C protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for PDE1C Protein (His-GST) (ABIN7197331)

Target: PDE1C (phosphodiesterase 1C, Calmodulin-Dependent 70kDa (PDE1C))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 85 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This PDE1C protein is labelled with His-GST.

Purpose: Recombinant Human PDE1C Protein (His & GST Tag)

Sequence: Met 1-Glu634

Characteristics: A DNA sequence encoding the human PDE1C (Q8TAE4) (Met1-Glu634) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol, 3 mM DTT
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: PDE1C (phosphodiesterase 1C, Calmodulin-Dependent 70kDa (PDE1C))

Alternative Name: PDE1C

Background: Hcam3,cam-PDE1C,hCam-3,PDE1C belongs to the cyclic nucleotide phosphodiesterase family, PDE1 subfamily. Phosphodiesterases (PDEs) are a family of related phosphohydrolyases that selectively catalyze the hydrolysis of 3' cyclic phosphate bonds in adenosine and/or guanine 3',5' cyclic monophosphate (cAMP and/or cGMP). They regulate the cellular levels, localization and duration of action of these second messengers by controlling the rate of their degradation. PDEs are expressed ubiquitously, with each subtype having a specific tissue distribution. These enzymes are involved in many signal transduction pathways and their functions include vascular smooth muscle proliferation and contraction, cardiac contractility, platelet aggregation, hormone secretion, immune cell activation, and they are involved in learning and memory. PDE1C has a high affinity for both cAMP and cGMP. It is expressed in several tissues, including brain and heart. As a cyclic nucleotide phosphodiesterase, PDE1C has a dual-specificity for the second messengers cAMP and cGMP.

Molecular Weight:

Calculated MW: 100 kDa

Observed MW: 97 kDa

UniProt: Q8TAE4

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma