PMVK Protein (His tag)
Quick Overview for PMVK Protein (His tag) (ABIN7318901)
Target
See all PMVK ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This PMVK protein is labelled with His tag.
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Purpose
- Recombinant Human Phosphomevalonate Kinase/PMVK Protein (His Tag)
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Sequence
- Met 1-Leu192
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Characteristics
- Recombinant Human Phosphomevalonate Kinase is produced by our E.coli expression system and the target gene encoding Met1-Leu192 is expressed with a 6His tag at the N-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Phosphomevalonate Kinase (PMVK) (AA 1-192) protein (His tag)
custom-made PMVK Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Phosphomevalonate Kinase (PMVK) (AA 1-192) protein (His tag)custom-made PMVK Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Phosphomevalonate Kinase (PMVK) (AA 1-192) protein (His-IF2DI Tag)PMVK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Phosphomevalonate Kinase (PMVK) (AA 1-192) protein (GST tag)PMVK Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Phosphomevalonate Kinase (PMVK) protein (Myc-DYKDDDDK Tag)PMVK Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 10 % Glycerol, pH 7.5.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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Expiry Date
- 6 months
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- PMVK (Phosphomevalonate Kinase (PMVK))
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Alternative Name
- Phosphomevalonate Kinase/PMVK
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Background
- PMKI,PMKase,PMVK,Phosphomevalonate Kinase,hPMK,Phosphomevalonate kinase (PMVK) is a cytosolic enzyme. PMVK can be highly expressed in the heart,skeletal muscle, liver, pancreas, and kidney, it is expressed at lower levels in the brain, lung, and placenta. Induced by sterol, PMVK takes part in isopentenyl diphosphate biosynthesis through the mevalonate pathway. PMVK catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate in the fifth reaction of the cholesterol biosynthetic pathway.
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Molecular Weight
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Calculated MW: 24.2 kDa
Observed MW: 26 kDa
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Gene ID
- 10654
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UniProt
- Q15126
Target
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