PDGF-BB Homodimer (Active) protein (His tag)
Quick Overview for PDGF-BB Homodimer (Active) protein (His tag) (ABIN7197388)
Target
Protein Type
Biological Activity
Origin
Source
Purity
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Purification tag / Conjugate
- His tag
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Purpose
- Recombinant Human PDGF-BB Protein (His Tag)(Active)
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Sequence
- Ser82-Thr190
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Characteristics
- A DNA sequence encoding the human PDGFB (NP_148937.1) (Ser82-Thr190) was expressed with a polyhistidine tag at the N-terminus.
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Biological Activity Comment
- Measured by its binding ability in a functional ELISA.2.Immobilized human PDGFB at 10μg/mL (100μL/well) can bind human PDGFRβ-Fch, the EC50 of human PDGFRβ-Fch is 0.05-0.2 μg/mL.
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PDGF-BB Homodimer protein
Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 98 % as determined by reducing SDS-PAGE.
PDGF-BB Homodimer (AA 82-190) (Active) proteinOrigin: Human Host: Escherichia coli (E. coli) Recombinant >90 % as determined by reducing (R) and non-reducing (NR) SDS-PAGE. Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please refer to the printed manual for detailed information.
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Buffer
- Lyophilized from sterile PBS, 25 % Glycerol, pH 7.4
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- PDGF-BB Homodimer
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Alternative Name
- PDGF-BB
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Background
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Background: Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF/VEGF growth factor family. Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.Binding of PDGFB to its receptor elicits a variety of cellular responses. In addition, PDGFB is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.
Synonym: Platelet-Derived Growth Factor Subunit B, PDGF Subunit B, PDGF-2, Platelet-Derived Growth Factor B Chain, Platelet-Derived Growth Factor Beta Polypeptide, Proto-Oncogene c-Sis, Becaplermin, PDGFB, PDGF2, SIS
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Molecular Weight
- 14.3 kDa
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NCBI Accession
- NP_148937
Target
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