PARP3 Protein (His-GST)

Catalog No. ABIN7317627

Recombinant PARP3 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for PARP3 Protein (His-GST) (ABIN7317627)

Target: PARP3 (Poly (ADP-Ribose) Polymerase Family, Member 3 (PARP3))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 85 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This PARP3 protein is labelled with His-GST.

Purpose: Recombinant Human PARP-3/PARP3 Protein (His & GST Tag)

Sequence: Met 1-Leu 533

Characteristics: A DNA sequence encoding the C-terminal segment of human PARP3 isoform 1 (Q9Y6F1-1) (Met 1-Leu 533) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: PARP3 (Poly (ADP-Ribose) Polymerase Family, Member 3 (PARP3))

Alternative Name: PARP-3/PARP3

Background: ADPRT3,ADPRTL2,ADPRTL3,ARTD3,IRT1,PADPRT-3,Regenerating islet-derived protein 4, also known as REG-like protein, REG4, GISP and RELP, a member of the regenerating gene family belonging to the calcium (C-type) dependent lectin superfamily, has been found to be involved in malignancy in several different organs including the stomach, colorectum, pancreas and prostate. It is highly expressed in the gastrointestinal tract and markedly up-regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and inflammatory bowel disease. Expression of the Reg4 in different cell types has been associated with regeneration, cell growth and cell survival, cell adhesion and resistance to apoptosis. REG4 protein overexpression is associated with an unfavorable response to preoperative chemoradiotherapy and may be used as a predictive biomarker clinically. REG4 may play an important role in the development and progression of colorectal cancer, as well as in intestinal morphogenesis and epithelium restitution.

Molecular Weight:

Calculated MW: 87.8 kDa

Observed MW: 95 kDa

Gene ID: 10039

UniProt: Q9Y6F1