RHEB Protein (GST tag)
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- Target See all RHEB Proteins
- RHEB (Ras Homolog Enriched in Brain (RHEB))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This RHEB protein is labelled with GST tag.
- Purpose
- Recombinant Human RHEB Protein (GST Tag)
- Sequence
- Met1-Met184
- Characteristics
- Recombinant Human RHEB is produced by our E.coli expression system and the target gene encoding Met1-Met184 is expressed with a GST tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Ras Homolog Enriched in Brain (RHEB) (AA 1-184) protein (His tag)
RHEB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Ras Homolog Enriched in Brain (RHEB) (AA 1-181) protein (T7 tag)RHEB Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Ras Homolog Enriched in Brain (RHEB) (AA 1-184) protein (GST tag)RHEB Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Ras Homolog Enriched in Brain (RHEB) protein (Myc-DYKDDDDK Tag)RHEB Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 10 mM GSH, pH 8.0.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- RHEB (Ras Homolog Enriched in Brain (RHEB))
- Alternative Name
- RHEB (RHEB Products)
- Background
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Background: GTP-Binding Protein Rheb (RHEB) is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. Highest levels of RHEB can be found in the skeletal and cardiac muscle, and it is vital in the regulation of growth and cell cycle progression due to its role in the Insulin/TOR/S6K signaling pathway. RHEB stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling, and it activates the protein kinase activity of mTORC1. RHEB has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, farnesylation of the protein is required for this activity.
Synonym: GTP-Binding Protein Rheb, Ras Homolog Enriched in Brain, RHEB, RHEB2
- Molecular Weight
- 20.4 kDa
- UniProt
- Q15382
- Pathways
- RTK Signaling
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