RHEB Protein (His tag)
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- Target See all RHEB Proteins
- RHEB (Ras Homolog Enriched in Brain (RHEB))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This RHEB protein is labelled with His tag.
- Purpose
- Recombinant Human RHEB Protein (His Tag)
- Sequence
- Met 1-Cys181
- Characteristics
- A DNA sequence encoding the human RHEB (Q15382)(Met1-Cys181) was fused with a polyhistide tag at the N-terminus.
- Purity
- > 85 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product RHEB Protein
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Ras Homolog Enriched in Brain (RHEB) (AA 1-184) protein (His tag)
RHEB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Ras Homolog Enriched in Brain (RHEB) (AA 1-181) protein (T7 tag)RHEB Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Ras Homolog Enriched in Brain (RHEB) (AA 1-184) protein (GST tag)RHEB Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Ras Homolog Enriched in Brain (RHEB) (AA 1-181) protein (His tag)Crystallography grade RHEB Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Ras Homolog Enriched in Brain (RHEB) (AA 1-181) protein (His tag)Crystallography grade RHEB Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Ras Homolog Enriched in Brain (RHEB) protein (Myc-DYKDDDDK Tag)RHEB Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- RHEB (Ras Homolog Enriched in Brain (RHEB))
- Alternative Name
- RHEB (RHEB Products)
- Synonyms
- RHEB2 Protein, RHEB Protein, rheb2 Protein, CG1081 Protein, DReb Protein, Dm Rheb Protein, DmRheb Protein, Dmel\CG1081 Protein, Q9VND8 Protein, RheB Protein, dRheb Protein, rheb Protein, Ras homolog, mTORC1 binding Protein, Ras homolog enriched in brain Protein, ras homolog enriched in brain Protein, Ras homolog, mTORC1 binding L homeolog Protein, RHEB Protein, Rheb Protein, rheb Protein, rheb.L Protein
- Background
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Background: RHEB is a recently discovered member of the Ras superfamily that may be involved in neural plasticity. This function is novel and not typically associated with the Ras proteins. RHEB gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. RHEB is vital in regulation of growth and cell cycle progression due to its role in the insulin / TOR / S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of RHEB is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22.
Synonym: GTP-Binding Protein Rheb, Ras Homolog Enriched in Brain, RHEB, RHEB2
- Molecular Weight
- 22.4 kDa
- UniProt
- Q15382
- Pathways
- RTK Signaling
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