ERBB3 Protein (His tag)

Catalog No. ABIN7320073

Recombinant ERBB3 protein expressed in HEK-293 Cells.

Quick Overview for ERBB3 Protein (His tag) (ABIN7320073)

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 93 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This ERBB3 protein is labelled with His tag.

Purpose: Recombinant Mouse HER3/ErbB3 Protein (His Tag)(Active)

Sequence: Met 1-His 641

Characteristics: A DNA sequence encoding the mouse ERBB3 (Q61526) extracellular domain (Met 1-His 641) was expressed, with a C-terminal polyhistidine tag.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA.1. Immobilized mouse ErbB3-His at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1 (isoform Beta1) with a linear range of 0.018-0.125 μg/ml.2. Immobilized mouse ErbB3-His at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1 (aa 2-246)-Fc with a linear range of 6.25-100 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Alternative Name: HER3/ErbB3

Background:

Background: ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: C76256;Erbb-3;Erbb-3r;Erbb3;Erbb3r;Her3

Molecular Weight: 70 kDa

UniProt: Q61526

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway