Ret Proto-Oncogene Protein (RET) (AA 658-1114) (His-GST)

Catalog No. ABIN7317056

This Recombinant Ret Proto-Oncogene protein is produced in Baculovirus infected Insect Cells.

Quick Overview for Ret Proto-Oncogene Protein (RET) (AA 658-1114) (His-GST) (ABIN7317056)

Target: Ret Proto-Oncogene (RET)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 658-1114

Purification tag / Conjugate: This Ret Proto-Oncogene protein is labelled with His-GST.

Purpose: Recombinant Human RET Kinase Protein (aa 658-1114, His & GST Tag)

Sequence: His 658-Ser 1114

Characteristics: A DNA sequence encoding the cytoplasmic domain of human RET (P07949-1) (His 658-Ser 1114) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 17 nmol/min/mg using synthetic TRK-C-derived Peptide (R11-VYSTDYYRLFNPS) as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: Ret Proto-Oncogene (RET)

Alternative Name: RET Kinase

Background: CDHF12,CDHR16,HSCR1,MEN2A,MEN2B,MTC1,PTC,RET-ELE1,RET51,RET proto-oncogene, also known as RET, is a cell-surface molecule that transduce signals for cell growth and differentiation. It contains 1 cadherin domain and 1 protein kinase domain. RET proto-oncogene belongs to the protein kinase superfamily, tyr protein kinase family. RET proto-oncogene is involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. It phosphorylates PTK2/FAK1 and regulates both cell death/survival balance and positional information. RET is required for the molecular mechanisms orchestration during intestine organogenesis, involved in the development of enteric nervous system and renal organogenesis during embryonic life, promotes the formation of Peyer's patch-like structures, modulates cell adhesion via its cleavage, involved in the development of the neural crest. RET proto-oncogene is active in the absence of ligand, triggering apoptosis. RET acts as a dependence receptor, in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. It also regulates nociceptor survival and size, triggers the differentiation of rapidly adapting (RA) mechanoreceptors, mediated several diseases such as neuroendocrine cancers.

Molecular Weight:

Calculated MW: 76.7 kDa

Observed MW: 70 kDa

Gene ID: 5979

UniProt: P07949

Pathways: RTK Signaling, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation