Reticulon 4 Protein (RTN4) (GST tag)

Catalog No. ABIN7317322

This Recombinant Reticulon 4 protein is expressed in Escherichia coli (E. coli).

Quick Overview for Reticulon 4 Protein (RTN4) (GST tag) (ABIN7317322)

Target: Reticulon 4 (RTN4)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 70 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Reticulon 4 protein is labelled with GST tag.

Purpose: Recombinant Human RTN4/NOGO-A Protein (GST Tag)

Sequence: Met 1-Val 185

Characteristics: A DNA sequence encoding the human RTN4 (NP_065393.1) N-terminal fragment (Met 1-Val 185) was fused with the GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 150 mM NaCl, 1 mM DTT, 0.2 mM GSH, pH 7.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Reticulon 4 (RTN4)

Alternative Name: RTN4/NOGO-A

Background: ASY,DGU,DKFZp781L1143,HIGM4,NI220/250,NOGO,NOGO-A,NOGOC,NSP,NSP-CL,Nbla00271,Nbla10545,Nogo-B,Nogo-C,RTN-X,RTN4-A,RTN4-B1,RTN4-B2,RTN4-C,UDG,UNG1,UNG15,UNG2,Reticulon-4, also known as Foocen, Neurite outgrowth inhibitor, Nogo protein, Neuroendocrine-specific protein, Neuroendocrine-specific protein C homolog, RTN-x, Reticulon-5 and RTN4, is a multi-pass membrane protein which contains onereticulon domain. Isoform1of RTN4 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform2 of RTN4 is widely expressed except for the liver. Isoform3of RTN4 is expressed in brain, skeletal muscle and adipocytes. Isoform4of RTN4 is testis-specific. Reticulon-4 / RTN4 is a developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Reticulon-4 / RTN4 regulates neurite fasciculation, branching and extension in the developing nervous system. Reticulon-4 / RTN4 is involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. It regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. Isoform 2 of RTN4 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 of RTN4 inhibit BACE1 activity and amyloid precursor protein processing.

Molecular Weight:

Calculated MW: 46.2 kDa

Observed MW: 48 kDa

Gene ID: 57142

NCBI Accession: NP_065393

UniProt: Q9NQC3

Pathways: Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome