SMAD2 Protein (AA 2-467) (His-GST)

Catalog No. ABIN7320169

Recombinant SMAD2 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for SMAD2 Protein (AA 2-467) (His-GST) (ABIN7320169)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Protein Type: Recombinant

Origin: Mouse

Source: Baculovirus infected Insect Cells

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 2-467

Purification tag / Conjugate: This SMAD2 protein is labelled with His-GST.

Purpose: Recombinant Mouse SMAD2 Protein (His & GST Tag)

Sequence: Ser2-Ser467

Characteristics: A DNA sequence encoding the mouse SMAD2 (Q62432-Isoform Long) (Ser2-Ser467) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM Nacl, pH 8.0, 10 % glycerol, 3 mM DTT
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Alternative Name: SMAD2

Background: 7120426M23Rik,Madh2,Madr2,Smad-2, mMad2,SMAD2 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD2 mediates the signal of the TGF-beta, and therefore regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. SMAD2 is the downstream signal transducers of TGF-beta-1 in human dental pulp cells. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. Phosphorylated SMAD2 is able to form a complex with SMAD4 or SARA. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression.

Molecular Weight:

Calculated MW: 80.0 kDa

Observed MW: 90 kDa

UniProt: Q62432

Pathways: Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus