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SMAD3 Protein (GST tag,His tag)

This Recombinant SMAD3 protein is expressed in Baculovirus infected Insect Cells.
Catalog No. ABIN7320082

Quick Overview for SMAD3 Protein (GST tag,His tag) (ABIN7320082)

Target

See all SMAD3 Proteins
SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Protein Type

Recombinant

Origin

  • 12
  • 3
  • 2
  • 1
  • 1
Mouse

Source

  • 14
  • 3
  • 1
  • 1
Baculovirus infected Insect Cells

Purity

> 85 % as determined by SDS-PAGE
  • Purification tag / Conjugate

    This SMAD3 protein is labelled with GST tag,His tag.

    Purpose

    Recombinant Mouse SMAD3 Protein (His & GST Tag)

    Sequence

    Met1-Ser425

    Characteristics

    A DNA sequence encoding the mouse SMAD3 (NP_058049.3) (Met1-Ser425) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus. Mouse, Human and Rat sequences are identical.

    Endotoxin Level

    < 1.0 EU per μg of the protein as determined by the LAL method.
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  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Please refer to the printed manual for detailed information.

    Buffer

    Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 2 mM GSH, 10 % glycerol, pH 7.4

    Storage

    4 °C,-20 °C,-80 °C

    Storage Comment

    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • Target

    SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

    Alternative Name

    SMAD3

    Background

    Background: SMAD3 belongs to the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD3 is involved in cell signalling. It modulates signals of activin and TGFβ's. Binding of SMAD3 with SMAD4 enables its transmigration into the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD3 is a receptor-regulated SMAD (R-SMAD). In mice, mutation of SMAD3 has been linked to colorectal adenocarcinoma, increased systemic inflammation, and accelerated wound healing. Increased SMAD3 activity has been implicated in the pathogenesis of scleroderma. Smad3 is also a multifaceted regulator in adipose physiology and the pathogenesis of obesity and type 2 diabetes.

    Synonym: AU022421,Madh3

    Molecular Weight

    75.9 kDa

    UniProt

    P84025

    Pathways

    Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
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