SMAD3 Protein (GST tag,His tag)
Quick Overview for SMAD3 Protein (GST tag,His tag) (ABIN7320082)
Target
See all SMAD3 ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This SMAD3 protein is labelled with GST tag,His tag.
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Purpose
- Recombinant Mouse SMAD3 Protein (His & GST Tag)
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Sequence
- Met1-Ser425
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Characteristics
- A DNA sequence encoding the mouse SMAD3 (NP_058049.3) (Met1-Ser425) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus. Mouse, Human and Rat sequences are identical.
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please refer to the printed manual for detailed information.
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Buffer
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 2 mM GSH, 10 % glycerol, pH 7.4
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
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Alternative Name
- SMAD3
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Background
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Background: SMAD3 belongs to the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD3 is involved in cell signalling. It modulates signals of activin and TGFβ's. Binding of SMAD3 with SMAD4 enables its transmigration into the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD3 is a receptor-regulated SMAD (R-SMAD). In mice, mutation of SMAD3 has been linked to colorectal adenocarcinoma, increased systemic inflammation, and accelerated wound healing. Increased SMAD3 activity has been implicated in the pathogenesis of scleroderma. Smad3 is also a multifaceted regulator in adipose physiology and the pathogenesis of obesity and type 2 diabetes.
Synonym: AU022421,Madh3
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Molecular Weight
- 75.9 kDa
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UniProt
- P84025
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Pathways
- Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
Target
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