SMAD3 Protein (His tag,DYKDDDDK Tag)
Quick Overview for SMAD3 Protein (His tag,DYKDDDDK Tag) (ABIN7318786)
Target
See all SMAD3 ProteinsProtein Type
Origin
Source
Purity
-
-
Purification tag / Conjugate
- This SMAD3 protein is labelled with His tag,DYKDDDDK Tag.
-
Purpose
- Recombinant Human SMAD3 Protein (His & Flag Tag)
-
Sequence
- Ser2-Ser425
-
Characteristics
- Recombinant Human Mothers Against Decapentaplegic Homolog 3 is produced by our E.coli expression system and the target gene encoding Ser2-Ser425 is expressed with a 6His, Flag tag at the N-terminus.
-
Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
-
-
Want other Options for this Protein ?
!Discover Our Predefined Custom Proteins and Custom Protein Services!Your project requires further customization? Contact us and discover our custom protein solutions
-
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 33-178) protein (His-IF2DI Tag)
SMAD3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-425) protein (His tag)SMAD3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
SMAD, Mothers Against DPP Homolog 3 (SMAD3) protein (GST tag,His tag)SMAD3 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 85 % as determined by SDS-PAGE
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 31-141) protein (GST tag)SMAD3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)SMAD3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD Active
-
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
- Please refer to the printed manual for detailed information.
-
Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB,500 mM NaCl, pH 7.5.
-
Storage
- 4 °C,-20 °C,-80 °C
-
Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
-
- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
-
Alternative Name
- SMAD3
-
Background
-
Background: Mothers against decapentaplegic homolog 3(SMAD3) is a cytoplasm protein which belongs to the dwarfin/SMAD family. Smad proteins undergo rapid nuclear translocation upon stimulation by transforming growth factor and in so doing transduce the signal into the nucleus. Receptor-regulated SMAD is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. SMAD3 binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. It also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive.
Synonym: Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3, SMAD3, MADH3
-
Molecular Weight
- 50.3 kDa
-
UniProt
- P84022
-
Pathways
- Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
Target
-
