SPG21 Protein (His-GST)
Quick Overview for SPG21 Protein (His-GST) (ABIN7320147)
Target
See all SPG21 ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This SPG21 protein is labelled with His-GST.
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Purpose
- Recombinant Mouse SPG21 Protein (His & GST Tag)
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Sequence
- Met 1-Pro 308
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Characteristics
- A DNA sequence encoding the mouse SPG21 isoform 1 (Q9CQC8-1) (Met 1-Pro 308) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Spastic Paraplegia 21 (SPG21) (AA 1-308) protein (His tag)
custom-made SPG21 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Spastic Paraplegia 21 (SPG21) (AA 1-308) protein (His tag)custom-made SPG21 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Spastic Paraplegia 21 (SPG21) (AA 1-308) protein (GST tag)SPG21 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Spastic Paraplegia 21 (SPG21) (AA 1-308) protein (His tag)SPG21 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS
Spastic Paraplegia 21 (SPG21) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)SPG21 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
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Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 3 mM DTT, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- SPG21 (Spastic Paraplegia 21 (SPG21))
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Alternative Name
- SPG21
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Background
- ACP33,BM-019,C78576,D9Wsu18e,GL010,MAST,Spastic paraplegia 21 (SPG21), also known as acid Cluster Protein 33 (ACP33) and Mast syndrome protein, is a member of the AB hydrolase superfamily. Human SPG21 is a 308 amino acid residue protein widely expressed in all tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. SPG21 binds to the hydrophobic C-terminal amino acids of CD4 which are involved in repression of T cell activation via the noncatalytic alpha/beta hydrolase fold domain. SPG21 thus is proposed to play a role as a negative regulatory factor in CD4-dependent T-cell activation of CD4. Defects in SPG21 are the cause of spastic paraplegia autosomal recessive type 21, also known as Mast syndrome, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. SPG21 is also associated with dementia and other central nervous system abnormalities.
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Molecular Weight
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Calculated MW: 62.8 kDa
Observed MW: 52 kDa
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UniProt
- Q9CQC8
Target
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