SPG21 Protein (GST tag)

Catalog No. ABIN7317839

Product Details

Target: SPG21 (Spastic Paraplegia 21 (SPG21))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This SPG21 protein is labelled with GST tag.

Purpose: Recombinant Human SPG21 Protein (GST Tag)

Sequence: Met 1-Gln 308

Characteristics: A DNA sequence encoding the full length of human SPG21 (NP_057714.1) (Met 1-Gln 308) was expressed with the GST tag at the N-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SPG21 (Spastic Paraplegia 21 (SPG21))

Alternative Name: SPG21 (SPG21 Products)

Synonyms: ACP33 Protein, GL010 Protein, MAST Protein, BM-019 Protein, C78576 Protein, D9Wsu18e Protein, Maspardin Protein, wu:fd07h02 Protein, zgc:73091 Protein, SPG21, maspardin Protein, spastic paraplegia 21 (autosomal recessive, Mast syndrome) Protein, SPG21, maspardin S homeolog Protein, SPG21 Protein, Spg21 Protein, spg21.S Protein, spg21 Protein

Background:

Background: Spastic paraplegia 21 (SPG21), also known as acid Cluster Protein 33 (ACP33) and Mast syndrome protein, is a member of the AB hydrolase superfamily. Human SPG21 is a 308 amino acid residue protein widely expressed in all tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. SPG21 binds to the hydrophobic C-terminal amino acids of CD4 which are involved in repression of T cell activation via the noncatalytic alpha/beta hydrolase fold domain. SPG21 thus is proposed to play a role as a negative regulatory factor in CD4-dependent T-cell activation of CD4. Defects in SPG21 are the cause of spastic paraplegia autosomal recessive type 21, also known as Mast syndrome, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. SPG21 is also associated with dementia and other central nervous system abnormalities.

Synonym: ACP33,BM-019,GL010,MAST

Molecular Weight: 61 kDa

NCBI Accession: NP_057714